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在常温(28℃)下用稀硫酸、氢氧化钠、氨水、过氧化氢溶液处理稻草原料,以实际糖化率为衡量指标对预处理效果进行比较。结果表明2%氢氧化钠溶液预处理效果最好。稻草原料通过温和的碱预处理后,绝大部分木质素被去除,但仍然有超过一半的半纤维素残留。以康宁木霉(T.koningii)QF-02生产的复合酶比两种商品纤维素酶制剂能更有效酶解糖化碱预处理稻草。自制复合酶的最适温度和最适pH分别为50℃和4.8;考虑酶解效率和操作费用,酶解时间48h、酶载量10FPU/g稻草、底物浓度8%(w/v)是合理的选择。
The raw materials of straw were treated with dilute sulfuric acid, sodium hydroxide, ammonia and hydrogen peroxide solution at normal temperature (28 ℃). The actual saccharification rate was used as a measure to compare the pretreatment results. The results show that 2% sodium hydroxide solution pretreatment best. Most of the lignin is removed after the straw material has been pretreated with a mild base, but more than half of the hemicellulose remains. The complex enzyme produced by T.koningii QF-02 was able to enzymatically hydrolyze saccharified alkali pretreated straw more efficiently than both commercial cellulase preparations. The optimum temperature and optimum pH of self-made complex enzyme were 50 ℃ and 4.8, respectively. Considering the efficiency of enzymolysis and operation cost, the enzymolysis time was 48h, the enzyme loading was 10 FPU / g straw and the substrate concentration was 8% (w / v) Reasonable choice.