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目的探讨肺癌组织中脆性组氨酸三聚体(Fragile Histidine Triad,FHIT)和Raf激酶抑制蛋白(Raf Kinase Inhibitor Protein,RKIP)的表达及其与临床病理特征的关系。方法应用免疫组织化学方法检测132例肺癌组织及相应癌旁正常肺组织中FHIT与RKIP蛋白的表达情况,分析两者与肺癌临床病理学参数的关系。结果肺癌组织中FHIT的阳性表达率为31.82%(42/132),低于正常肺组织的73.48%(97/132),差异有统计学意义(χ2=45.963,P<0.05)。肺鳞癌中FHIT的阳性表达率低于肺腺癌,差异有统计学意义(P<0.05),吸烟组FHIT的阳性表达率低于非吸烟组,差异有统计学意义(P<0.05)。肺癌组织中RKIP的阳性表达率为33.33%(44/132),低于正常肺组织的75.76%(100/132),差异有统计学意义(χ2=47.911,P<0.05)。RKIP的阳性表达率与病理类型及吸烟史无关(P>0.05)。FHIT及RKIP的表达水平与肺癌的分化程度、p TNM分期、淋巴结转移及术后生存时间均有明显关系(P<0.05);FHIT蛋白与RKIP蛋白的表达呈正相关(P<0.05)。结论 FHIT与RKIP的缺失在肺癌的发生、发展中起重要作用,RKIP及FHIT的低表达促进了肺癌细胞的侵袭和转移,两者的联合检测为肺癌的诊断及预后判断提供了重要的临床价值。
Objective To investigate the expression of Fragile Histidine Triad (FHIT) and Raf Kinase Inhibitor Protein (RKIP) in lung cancer and its relationship with clinicopathological features. Methods The expression of FHIT and RKIP protein in 132 cases of lung cancer and adjacent normal lung tissues was detected by immunohistochemistry. The relationship between them and clinicopathological parameters of lung cancer was analyzed. Results The positive expression rate of FHIT in lung cancer tissues was 31.82% (42/132), which was lower than that in normal lung tissues (73.48%, 97/132) (χ2 = 45.963, P <0.05). The positive rate of FHIT in lung squamous cell carcinoma was lower than that in lung adenocarcinoma (P <0.05). The positive rate of FHIT in smoking group was lower than that in non-smoking group (P <0.05). The positive expression rate of RKIP in lung cancer was 33.33% (44/132), which was lower than 75.76% (100/132) in normal lung tissue, the difference was statistically significant (χ2 = 47.911, P <0.05). The positive expression rate of RKIP had no correlation with pathological type and smoking history (P> 0.05). The expression levels of FHIT and RKIP were significantly correlated with the degree of differentiation, p TNM stage, lymph node metastasis and postoperative survival time (P <0.05). There was a positive correlation between FHIT protein and RKIP protein expression (P <0.05). Conclusion The deletion of FHIT and RKIP plays an important role in the occurrence and development of lung cancer. The low expression of RKIP and FHIT promotes the invasion and metastasis of lung cancer cells. The combined detection of these two genes provides important clinical value for the diagnosis and prognosis of lung cancer. .