First detection of Leishmania infantum DNA in wild caught Phlebotomus papatasi in endemic focus of c

• 作 者: Rassi Yavar Karami Hadi Abai Mohammad Reza M Mohebali Bakshi Hasan Oshaghi Mohammad Ali Rafizadeh Sina Bagherpoor Hagigi Habib Hosseini Abodolrahim Gholami Manuchehr
• 机 构: Department of Medical Entomology&Vector Control, School of Public Health, Tehran University of Medic
• 出 处: 亚太热带生物医学杂志（英文版）
• 发表日期: 2013年10期
• 关键词: Leishmania infantum Leishmania major Vector Reservoir Iran

Objective:To identify the vectors and reservoirs of cutaneous leishmaniasis in the endemic focus of Farashband, Fars Province, South of Iran.
Methods: Sticky papers and Sherman trap were used for collection of sand flies and rodents, respectively. Polymerase chain reaction (PCR) of kDNA, ITS1-rDNA were used for identification of Leishmania parasite in sand flies as well as rodents.
Results:Totally 2 010 sand flies were collected and the species of Phlebotomus papatasi Scopoli was the common specimen in outdoors and indoors places. PCR technique was employed on 130 females of Phlebotomus papatasi. One of them (0.76%) was positive to parasite Leishmania major (L. major) and one specimen (0.76%) was positive to Leishmania infantum. Microscopic investigation on blood smear of the animal reservoirs for amastigote parasites revealed 16 (44%) infected Tatera indica. Infection of them to L. major was confirmed by PCR against kDNA loci of the parasite.
Conclusions: The results indicated that Phlebotomus papatasi was the dominant species circulating two species of parasites including L. major and Leishmania infantum among human and reservoirs. Furthermore, Tatera indica is the only main host reservoir for maintenance of the parasite source in the area.