论文部分内容阅读
目的探讨胰岛素干预治疗对骨骼肌细胞内脂肪酸转运蛋白表达的影响。方法建立高脂喂养联合小剂量STZ诱导的糖尿病SD大鼠模型,在FPG升高后3d予中效胰岛素或格列齐特(GLI)治疗3周,血糖控制目标随机血糖<8 mmol/L。Western blot检测骨骼肌细胞内脂蛋白脂酶(LPL),脂肪酸转位酶(FAT)、脂肪酸转运蛋白1(FATP-1)、脂肪酸结合蛋白(FABP)及肉毒碱棕榈酰转移酶1(CPT-1)蛋白表达水平。结果与NC组比较,DM组骨骼肌细胞内LPL蛋白表达上调30%,Ins组和GLI组治疗下调了LPL蛋白表达;DM组骨骼肌细胞内CPT-1和FAT蛋白分别下调了45%和47%,Ins组和GLI组治疗分别上调了FAT蛋白表达31%和26%,Ins组同时上调了CPT-1蛋白表达57.5%;FATP-1和FABP蛋白表达各组间差异无统计学意义。结论胰岛素治疗改善了细胞内脂肪酸转运及线粒体氧化,可能是骨骼肌细胞内脂质沉积减少的机制之一。
Objective To investigate the effect of insulin intervention on the expression of fatty acid transporters in skeletal muscle cells. Methods A rat model of diabetic SD induced by high-fat diet combined with low-dose STZ was established. The rats were treated with medium-acting insulin or gliclazide (GLI) for 3 weeks after the increase of FPG. The blood glucose control target was randomized to receive blood glucose <8 mmol / L. Western blot was used to detect the levels of lipoprotein lipase (LPL), fatty acid translocase (FAT), fatty acid transporter 1 (FATP-1), fatty acid binding protein (FABP) and carnitine palmitoyl transferase 1 -1) protein expression level. Results Compared with NC group, LPL protein expression in skeletal muscle cells was up-regulated by 30% in DM group and LPL protein expression was down-regulated in Ins group and GLI group. In DM group, CPT-1 and FAT protein were down-regulated by 45% and 47 %. Ins group and GLI group increased the expression of FAT protein by 31% and 26%, while Ins group also increased the expression of CPT-1 protein by 57.5%. There was no significant difference between the two groups in the expression of FATP-1 and FABP protein. Conclusion Insulin treatment improves intracellular fatty acid transport and mitochondrial oxidation, which may be one of the mechanisms of reduced lipid deposition in skeletal muscle cells.