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将冰冻干燥的日本血吸虫成虫用反复冰冻融解方法制成雌虫、雄虫及混合抗原。以混合抗原免疫家兔制备抗血清。电泳在特制的厚约2毫米琼脂平板上进行,条件为巴比妥缓冲液pH8.6,离子强度0.05,电流为每张玻片3毫安,电泳70分钟,置于37℃温箱中24小时再于室温下放置一日后观察沉淀线的出现,并进行了圆盘电泳和吸收试验。结果:混合抗原共出现11条沉淀线,包括阳极侧4
The lyophilized adult Schistosoma japonicum was made into female, male and mixed antigens by repeated freeze-thaw method. Rabbit was immunized with mixed antigen to prepare antiserum. Electrophoresis was performed on a specially prepared agar plate about 2 mm thick under the conditions of pH 8.6 with barbital buffer, ionic strength of 0.05, current of 3 mA per slide, electrophoresis for 70 minutes and placed in a 37 ° C incubator Hour and then placed at room temperature after a day to observe the emergence of precipitation lines, and the disk electrophoresis and absorption test. Results: A total of 11 precipitation lines appeared on the mixed antigen, including the anode side 4