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最近Hendricks等用培养方法对一批怀疑为皮肤利什曼病的病人的损害处分离原虫,其中有5例来自巴西、33例来自巴拿马。用NNN培养基只有7例巴拿马病人分离出原虫,但用Schneider氏Drosophila培养基从所有病人的损害处分离出了原虫。从而认为后一种培养基的效果比NNN培养基为好。Walton也认为培养巴西利什曼复合体的原虫以Schneider氏的培养好。本文报道了用有不同覆盖液的NNN培养基却得到了不同的结果:除在制作培养基时出现的冷凝水外,还用蒸馏水、0.9%盐水和Locke氏液作为覆盖液。这3种覆盖液与血琼脂的比为1/15。发现巴西的皮肤利什曼巴西亚种及从巴西的粘膜皮肤利什曼病人分离出的原虫在冷凝水和蒸馏水中的生长都非
Recently, Hendricks et al. Used culture methods to isolate protozoa from damage to a group of patients suspected of having cutaneous leishmaniasis, five of them from Brazil and 33 from Panama. Protozoans were isolated from only 7 Panama patients in NNN medium but protozoans were isolated from all patient lesions using Schneider’s Drosophila medium. Thus, the latter medium is considered to be more effective than NNN medium. Walton also believes that protozoa that cultivate the Brazilian Leishmanian complex are cultured in Schneider’s. This paper reports that different results were obtained with NNN media with different cover solutions: distilled water, 0.9% saline and Locke’s solution as the cover solution, except for the condensed water that was present in the media. The ratio of these three coverings to blood agar was 1/15. It has been found that the parasites of the Brazilian Leishmania brasiliensis isolate and the mucosal cutaneous leishmaniasis in Brazil grew in both condensed water and distilled water