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近年来认为用精子抗原免疫人,特别是妇女,可能是调节生育的一条有效途径。因此,研究能使妇女产生免疫应答的精子抗原的性质以及这些抗原与自身抗原的关系是很有意义的。本文作者利用去垢剂溶解、外源凝集素亲和层析和用人同族抗血清进行间接免疫沉淀反应等技术,分离出一种人精子膜同族抗原(同族抗原在此处特指对女性具有免疫原性的男性特殊组织成分),并通过 SDS 聚丙烯酰胺凝胶电泳 SDS-PAGE)鉴定了该抗原的性质。方法是先对人精子(来自供者)进行氚(~3H)标记,然后用15mM 的脱氧胆酸盐将精子溶解,并
In recent years, it is considered that immunization of human beings with sperm antigens, especially women, may be an effective way to regulate fertility. Therefore, it is of interest to study the properties of sperm antigens that enable women to develop an immune response and the relationship of these antigens to self antigens. The authors isolated a human sperm membrane cognate antigen using detergent solubilization, lectin affinity chromatography, and indirect immunoprecipitation with human antisera (clathra antigen is herein specifically meant to be immunized against the female Virulence of male specific tissue components) and characterization of the antigen by SDS polyacrylamide gel electrophoresis SDS-PAGE. The method involves tritium (~ 3H) labeling of human spermatozoa (from a donor) and then the spermatozoa are solubilized with 15 mM deoxycholate