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黄芩带节茎段培养在MS+6-BA1mg/L+NAA0.2mg/L的培养基上,先诱导形成愈伤组织,然后出现许多绿色小芽。进一步移至MS培养基上产生大量的丛生芽。丛生芽培养干1/2MS+IBA0.5mg/L培养基上可诱导生根,形成完整植株。
The stem segments of Astragalus membranaceus cultured on the medium of MS+6-BA1mg/L+NAA 0.2mg/L first induced the formation of callus, and then appeared many green shoots. Move further to MS medium to produce a large number of clustered shoots. Clustered shoots were cultured on dry 1/2MS + IBA 0.5 mg/L medium to induce rooting and form complete plants.