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三氟乙酸(TFA)系氟烷的体内代谢产物,因具高度挥发性及水溶性而使提取困难,GLC 的衍生化反应多数亦须在无水条件下进行、难以采用。本文介绍利用相转移催化剂18-冠醚-6(Ⅰ)提取血或尿样中的TFA,制成4-溴甲基-7-甲氧基香豆素酯(Ⅱ)萤光衍生物,然后进行HPLC 法测定。操作:取血浆或尿样0.5ml,加0.007M(Ⅰ)的乙腈溶液250μl,蛋白质沉淀,而TFA 则与(Ⅰ)和溶液中存在的K~+或Na~+的络合物形成离子对留在溶液中。以5MHCl100μl 酸化使TFA 成非离解形式。加入干燥丙酮10ml,离心、得均一液相,由于溶剂化作
Trifluoroacetic acid (TFA) system of halothane metabolites, because of their high volatility and water solubility make extraction difficult, GLC most of the derivatization reaction must also be carried out under anhydrous conditions, it is difficult to use. This article describes the use of 18-crown-6 (Ⅰ) phase transfer catalyst to extract blood or urine samples of TFA, 4-bromomethyl-7-methoxy coumarin ester (Ⅱ) fluorescent derivatives, and then HPLC determination. Operation: Take 0.5 ml of plasma or urine sample, add 250 μl of 0.007 M (I) acetonitrile solution and precipitate the protein, while TFA forms ion pairs with (I) and K + or Na + complexes present in the solution Stay in solution. Acidification with 5 M HC1 100 μl rendered TFA in a non-dissociated form. Add dry acetone 10ml, centrifuged to obtain a homogeneous liquid phase, due to solvation