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为了了解小麦Glu-A3位点基因的等位变异对SDS沉降值的影响,我们对122个小麦品系的Glu-A3位点等位基因组成(分子标记),HMW亚基组成(聚丙烯酰胺凝胶电泳),以及这些小麦的品质参数SDS沉降值进行了鉴定,测量并进行了数据统计分析。方差分析显示,含有各Glu-A3等位基因的小麦品系SDS沉降值均值之间差异显著,Glu-A3位点基因的等位变异能解释SDS沉降值变异的9.09%,7个等位基因中含等位基因glu A3a,glu A3g和glu A3d的品系其平均沉降值都较高,而含glu A3b,glu A3f,和glu A3e的品系其平均沉降值都较低。不同HMW亚基背景下的分析结果也基本符合这个规律。等位基因glu A3e在地方品系中出现的频率很低,但是其中glu A3e所属品系的SDS沉降值均值显著低于所有材料沉降值均值;而在国外引进品种中出现频率最低的等位基因glu A3d,其所属材料SDS沉降值均值却显著大于所有材料的沉降值均值。这些特异的材料可以作为小麦品质改良的亲本,优质的等位基因可作为品质选育的指标。
In order to understand the effect of allelic variation of Glu-A3 loci on SDS sedimentation value, we investigated the allelic composition of Glu-A3 loci (molecular markers), HMW subunit composition (polyacrylamide gel Gel electrophoresis) and SDS sedimentation values of the quality parameters of these wheat were identified, measured and the data were statistically analyzed. An analysis of variance showed that there was significant difference in mean SDS sedimentation values for all wheat lines containing Glu-A3 alleles. Allelic variation in Glu-A3 loci could account for 9.09% variation in SDS sedimentation value among 7 alleles The strains with the alleles glu A3a, glu A3g and glu A3d had higher average sedimentation values, while the strains with glu A3b, glu A3f and glu A3e had lower average sedimentation values. The analysis results of different HMW subunits also basically accord with this rule. The frequency of allele glu A3e appeared in local lines is very low, but the average SDS sedimentation value of glu A3e strains was significantly lower than the average value of all material sedimentation values; while in the introduction of foreign varieties the lowest frequency allele glu A3d , The average value of the SDS sediment value belonging to its material is significantly greater than the average value of all the material sedimentation value. These specific materials can be used as parents for the improvement of wheat quality, and high-quality alleles can be used as indicators of quality breeding.