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1986—1990午先后对我国南方油菜产区10个省(市)的24个县,114块油菜地进行病毒病发病调查,采集病株标样进行种类鉴定。结果表明,油菜病毒病发生普遍,尤以武汉、长沙、成都和合肥等市的郊区发病最重。发病率都在30%以上。酶联免疫吸附和对流免疫电泳测定1122份样品的结果,各地病毒种类均以TuMV(芜菁花叶病毒)为主,占样品总数的80.48%[含其与CMV(黄瓜花叶病毒)和TMV(烟草花叶病毒)复合感染者],其他病毒单独侵染者较少。分别对4种不同类型油菜(甘蓝型、白菜型、芥菜型和其他类型)病株测定结果,也以TuMV侵染率高,约占测定样品数的52—98%。因此抗病育种应着重选育抗TuMV的品种。
From 1986 to 1990, we investigated the incidence of virus diseases in 24 counties and 114 rapeseed areas in 10 provinces (cities) in the southern rapeseed producing area in our country, and collected the samples of diseased plants for species identification. The results showed that the occurrence of rape virus disease is widespread, especially in the outskirts of Wuhan, Changsha, Chengdu and Hefei, the most serious. The incidence rate is above 30%. Enzyme-linked immunosorbent assay (ELISA) and convective immunity (IFA) were used to determine the results of 1122 samples. TuMV (Turnip Mosaic Virus) was the dominant virus in all the samples, accounting for 80.48% of the total samples [including CMV (cucumber mosaic virus) (Tobacco mosaic virus) co-infected], other viruses alone less infected. The results of the determination of four different types of rape (Brassica campestris, Brassica campestris, Brassica campestris and other types) also showed that the TuMV infection rates were high, accounting for 52-98% of the total number of the tested samples. Therefore, disease-resistant breeding should focus on breeding anti-TuMV varieties.