论文部分内容阅读
目的:探讨三氧化二砷(As2O3))对体外培养的小鼠气道成纤维细胞(FB)增殖及原癌基因c-myc与c-sis表达的影响。方法:按不同药物分7组,在体外培养的FB中分别加入浓度为0·25、0·5、1·0、2·0和4·0μmol/L的As2O3,在1、3、5和7d后用记数法观察FB生长情况,并用流式细胞仪(FCM)检测不同浓度药物对各组FB增殖的影响及各组c-myc与c-sis的阳性表达率。结果:各种实验浓度的As2O3对FB均有抑制作用,并有时间剂量效应。流式细胞仪分析显示,随着浓度的增高,G1期细胞比例逐渐增高,G2/M期细胞比例降低,浓度为2·0和4·0μmol/L的As2O3能显著抑制c-myc与c-sis的表达。结论:As2O3能抑制FB的增生,其机理可能与其下调c-myc与c-sis的表达有关。
Objective: To investigate the effects of arsenic trioxide (As2O3) on the proliferation of mouse airway fibroblasts (FB) cultured in vitro and the expression of protooncogene c-myc and c-sis. Methods: According to different drugs divided into 7 groups, in the in vitro cultured FB were added As0.25, 0.5, 1.0, 2.0 and 4.0 μmol / L of As2O3, in 1,3,5 and After 7 days, the growth of FB was observed by counting method. The effects of different concentrations of drugs on FB proliferation and the positive expression rates of c-myc and c-sis in each group were detected by flow cytometry (FCM). Results: Various concentrations of As2O3 had inhibitory effect on FB with time-dose effect. Flow cytometry analysis showed that with the increase of concentration, the proportion of cells in G1 phase increased gradually and the proportion of cells in G2 / M phase decreased. As2O3 at concentrations of 2.0 and 4.0 μmol / L significantly inhibited the expression of c-myc and c- sis expression. Conclusion: As2O3 can inhibit the proliferation of FB, the mechanism may be related to its down-regulation of c-myc and c-sis expression.