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目的观察极低频电磁场(ELF-MF)与铝负荷对学习记忆行为的影响,并初步探讨其可能机制。方法 64只雄性SPF级昆明小鼠每天接受50 Hz、2 mT ELF-MF暴露和(或)灌胃给予葡萄糖酸铝(200 mg Al3+/kg),一周6 d,共8周。8周后取大脑组织做HE染色观察小鼠脑组织病理形态变化;Morris水迷宫检测小鼠的学习记忆行为;生化方法检测小鼠大脑组织及血清中SOD活性和MDA含量。结果 ELF-MF暴露与铝负荷单独或联合暴露后小鼠的逃避潜伏期明显增长,在目标象限的停留时间比明显减少,各组与对照组相比均有显著性差异,而组间比较无显著性变化。病理切片结果显示,ELF-MF组、铝负荷组和ELF-MF+铝负荷组大脑皮质和海马区神经细胞和椎体细胞数目都出现减少,排列紊乱。通过氧化应激指标检测发现,二者均可致脑组织与血清中SOD活性明显降低以及MDA含量明显升高。结论 ELF-MF暴露与铝负荷单独或联合暴露均能够导致小鼠学习记忆障碍,其机制可能与氧化应激有关。
Objective To observe the effect of ELF-MF and aluminum load on learning and memory behaviors and to explore its possible mechanism. Methods Sixty-four male Kunming SPF Kunming mice were exposed to ELF-MF (50 Hz, 2 mT) and aluminum gluconate (200 mg Al3 + / kg) orally for 6 weeks a week for 8 weeks. After 8 weeks, the brain tissue was taken for HE staining to observe the pathological changes of brain tissue in mice. Morris water maze was used to detect the learning and memory behavior of mice. The biochemical methods were used to detect the activity of SOD and the content of MDA in brain tissue and serum. Results The escape latency of mice exposed to ELF-MF alone or in combination with aluminum stress increased significantly, and the residence time in the target quadrant decreased significantly compared with those in the control group, but there was no significant difference between the two groups Sexual change. Pathological sections showed that the number of neurons and vertebral cells in the cerebral cortex and hippocampus of ELF-MF group, aluminum-loaded group and ELF-MF + aluminum-loaded group both decreased and arranged disorderly. Oxidative stress index test found that both can cause brain tissue and serum SOD activity was significantly lower and MDA content was significantly increased. Conclusion Both ELF-MF exposure and aluminum stress alone or in combination can lead to learning and memory impairment in mice, which may be related to oxidative stress.