论文部分内容阅读
目的探索茶多酚(TP)在对抗石英粉尘氧化损伤中的作用,为石英粉尘毒作用的预防和控制工作提供参考。方法以外周静脉血红细胞膜谷胱甘肽过氧化物酶(GSH-px)活力、丙二醛(MDA)含量以及超氧化物歧化酶(T-SOD)活力为评价指标,以整群抽样法抽取接触石英粉尘的陶瓷厂健康一线接尘人员107名,抽取厂矿周围居民健康志愿者45名,分别采集静脉血2ml,检测两组人群在利用TP干预前后,红细胞膜SOD活力、MDA含量及GSH-px活力的变化。谷胱甘肽过氧化物酶(GSH-px)活力测定采用二硝基苯甲酸法,丙二醛(MDA)含量测定采用硫代巴比妥法(TBA法),超氧化物歧化酶(T-SOD)活力测定采用黄嘌呤氧化酶法,膜蛋白含量的测定采用考马斯亮蓝法。结果在TP干预后,接尘组人员红细胞膜SOD活力明显回升(P<0·05),MDA含量明显降低(P<0·05),GSH-px活力明显回升(P<0·05),在非接尘组人员中,SOD活力、MDA含量及GSH-px活力三指标变化不明显(P>0·05)。结论TP具有抗石英粉尘所致人红细胞膜脂质过氧化损伤作用。
Objective To explore the role of tea polyphenols (TP) in combating the oxidative damage of quartz dust and provide a reference for the prevention and control of quartz dust poisoning. Methods The glutathione peroxidase (GSH-px) activity, malondialdehyde (MDA) content and the activity of superoxide dismutase (T-SOD) in peripheral blood red blood cell membrane were evaluated by cluster sampling method In the ceramic factory contacted with quartz dust, 107 health workers were taken from the front line and 45 healthy volunteers were collected from the factories and mines. Two ml venous blood samples were collected. Before and after TP intervention, SOD activity, MDA content and GSH- px changes in vitality. The activity of glutathione peroxidase (GSH-px) was measured by dinitrobenzoate method. The content of malondialdehyde (MDA) was determined by TBA method, the activity of superoxide dismutase (T -SOD) activity determination using xanthine oxidase method, the determination of membrane protein content using Coomassie brilliant blue method. Results After intervention with TP, the activity of SOD in erythrocyte membrane increased significantly (P <0.05), the content of MDA decreased significantly (P <0.05) and the activity of GSH-px increased significantly (P <0.05) In non-exposed group, SOD activity, MDA content and GSH-px activity did not change significantly (P> 0.05). Conclusion TP possesses the anti-lipid peroxidation injury of erythrocyte membrane caused by quartz dust.