Preparation of glucose oxidase electrode containing hydrophobic silica nanoparticles by the sol-gel

• 作 者: 张琳 唐芳琼 袁金锁 江龙
• 机 构: Institute of Photographic Chemistry,Chinese Academy of Sciences,Beijing 100101,China,
• 出 处: Science in China(Series B)
• 发表日期: 1995年12期
• 关键词: hydrophobic biosensor silica preparing providing hexane ultrafine enzymatic reag

The influences of hydrophobic SiO_2 nanoparticles and the contents of glucose oxidase(GOD)upon the response current of enzyme electrode have been investigated by using the sol-gel method,in whichpolyvinylbutyryl(PVB)was used as a matrix.The influenoe of enzymatic activity was measured byelectrochemical method.Experimental data demonstrated that hydrophobic SiO_2 particles can immobilizeenzyme well,providing a good and simple method for preparing high quality GOD biosensor.The mechanismhas been discussed.

The influences of hydrophobic SiO 2 nanoparticles and the contents of glucose oxidase (GOD) upon the response current of enzyme enzyme electrodes have been investigated by using the sol-gel method, in which polyvinyl butyrate (PVB) was used as a matrix. The influence of enzymatic activity was measured by electrochemical method. Experimental data of the hydrophobic SiO 2 particles can immobilizeenzyme well, providing a good and simple method for preparing high quality GOD biosensor. The mechanism has been discussed.