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由致病疫霉Phytophthora infestans引起的晚疫病是马铃薯生产上最严重的病害之一,认识其群体结构特征,可为晚疫病防控策略的制定以及抗病品种的合理布局提供指导。对2009年采自宁夏一个种植有93个品种(品系)的马铃薯种质资源圃的致病疫霉进行了交配型、致病型和线粒体DNA单倍型分析,结果表明,116个致病疫霉菌株中存在A1、A2和自育型3种交配型,发生频率分别为24.1%、57.8%和18.1%,A2交配型为优势类型;对其中43个菌株的致病型进行测试,检测到两种致病类型:1.2.3.4.5.6.7.8.9.10.11和3.4.10,发生频率分别为95.3%和4.7%,可克服所有11个抗病基因的1.2.3.4.5.6.7.8.9.10.11类型占绝对优势;对62个菌株的线粒体DNA单倍型进行分析,检测到Ia和IIa两种类型,发生频率分别为74.2%和25.8%。综合表型和基因型数据分析发现,该马铃薯种质资源圃中致病疫霉群体致病型单一,但致病型毒力因子高度复合;线粒体DNA分析表明,该马铃薯种质资源圃引入了遗传背景较为复杂的致病疫霉“新”群体。
Late blight caused by Phytophthora infestans is one of the most serious diseases in potato production. To understand the characteristics of its population structure can provide guidance for the development of late blight prevention and control strategies and the rational distribution of resistant varieties. The mating type, pathogenicity and mitochondrial DNA haplotype analysis of Phytophthora infestans were carried out in a potato germplasm collected from 93 cultivars (lines) in Ningxia in 2009. The results showed that 116 pathogenic There were three mating types of A1, A2 and self-fertility in the mold strains, the frequency of which was 24.1%, 57.8% and 18.1%, respectively. A2 mating type was the predominant type. The pathogenicity of 43 strains was tested, Two types of pathogenicity: 1.2.3.4.5.6.7.8.9.10.11 and 3.4.10, with frequencies of 95.3% and 4.7%, respectively, can overcome 1.2.3.4.5.6.7.8.9.10 of all 11 disease resistance genes .11 types accounted for the absolute advantage; 62 strains of mitochondrial DNA haplotype analysis, detected Ia and IIa two types, the occurrence frequency was 74.2% and 25.8%. Comprehensive phenotypic and genotypic data analysis found that the potato germplasm resource groups in the pathogenicity of a single group of pathogenic virulence factors but highly complex; mitochondrial DNA analysis showed that the potato germplasm resources introduced The genetic background is more complex Phytophthora infestans “new ” groups.