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目的:探讨大肠癌ＣＤ４４ｖ６表达、ｐ(53)基因突变和染色体倍性与大肠癌转移之间的关系和作用机理。方法：流式细胞仪测定大肠癌细胞倍性及其在细胞周期各相中的分布；ＲＴ－ＰＣＲ方法及特异探针Ｄ３对大肠癌细胞进行Ｓｏｕｔｈｅｒｎ Ｂｌｏｔ，并对杂交条带进行辉度扫描；银染ＰＣＲ单链构象多态性（ＳＳＣＰ）技术检测大肠癌细胞ｐ(53)基因突变。结果：正常对照、良性腺瘤、肿瘤未转移和肿瘤转移组其ＣＤ４４ｖ６阳性率和ｐ(53)基因突变率呈逐渐上升趋势，ＣＤ４４ｖ６辉度扫描表明肿瘤未转移组（２３１７．２６±１９８．７３）和转移组（１００２４．１６±８５５．４０）相比较，表达量有显著差异（Ｐ＜０．０２）；ＣＤ４４ｖ６阳性和阴性两组中Ｇ２Ｍ期细胞均值分别占６．２４％和５．４８％（Ｐ＞０．０５），异倍体细胞分别为６２．１６％和６０．００％（Ｐ＞０．５）；而ｐ(53)基因突变和未突变两组中Ｇ２Ｍ细胞占１０．３３％和４．０１％（Ｐ＜０．０５），异倍体细胞分别为８５．２０％和３０，００％（Ｐ＜０．００５）；肿瘤异倍体细胞转移率显著高于二倍体细胞。结论：ＣＤ４４ｖ６的表达与转移高度相关，ＣＤ４４ｖ６和ｐ(53)基因突变与肿瘤转移关系可能涉及不同的作用机理。Ｃ?
Objective: To investigate the relationship between CD44v6 expression, p(53) gene mutation and ploidy of colorectal carcinoma and metastasis of colorectal cancer and its mechanism of action. Methods: Flow cytometry was used to measure the ploidy of colorectal cancer cells and their distribution in each phase of the cell cycle. RT-PCR method and specific probe D3 were used for Southern Blot of colorectal cancer cells, and the hybridization bands were scanned by luminance. Silver staining of single-stranded conformation polymorphisms (SSCP) technique for the detection of p (53) mutations in colorectal cancer cells. RESULTS: The positive rate of CD44v6 and the mutation rate of p(53) gene in the normal control, benign adenoma, tumor non-metastasis and tumor metastasis group showed a gradually increasing trend. The CD44v6 luminance scan showed no tumor metastasis group (2317.26±198.73). Compared with the metastatic group (10024.16±855.40), the expression levels were significantly different (P<0.02); the mean values of G2M phase cells in the CD44v6 positive and negative groups were 6.24% and 5.48, respectively. % (P>0.05), aneuploid cells were 62.16% and 60.00% respectively (P>0.5); while p(53) gene mutations and non-mutation were the two groups. 33% and 4.01% (P<0.05). Aneuploid cells were 85.20% and 30.00%, respectively (P<0.005). The metastasis rate of tumor aneuploid cells was significantly higher than twice. Somatic cells. Conclusion: The expression of CD44v6 is highly correlated with metastasis. The relationship between CD44v6 and p(53) mutations and tumor metastasis may involve different mechanisms. C?