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杂交方法是水稻杂交育种的关键环节之一。为了探索杂交方法的新路子,近年来,一些单位正在进行稻穗离体杂交试验,一般系采用温汤杀雄、营养液培养。这种方法较之常规的不离体杂交方法,有许多优点,但试验前需要准备一定设备和药品,配制营养液,因而应用时比较麻烦。为探索更简便易行的方法,笔者于1981年在四川省农科院水稻研究所实习时,进行了稻穗离体剪颖去雄杂交、田间或清水培养的试验。在此基础上,于1982年又在梁平、篷溪等地重复了该试验。在
Hybridization is one of the key aspects of hybrid rice breeding. In order to explore a new way of hybridization methods, in recent years, some units are conducting in vitro hybridization experiments of rice ear. Generally, they adopt Wen Tang Kill and nutrient solution culture. Compared with the conventional non-in vitro hybridization method, this method has many advantages. However, before the test, a certain amount of equipment and drugs are required to prepare the nutrient solution, which makes the application more troublesome. In order to explore a more simple and convenient method, the author in 1981 in Sichuan Academy of Agricultural Sciences Institute of Rice internship, carried out in vitro rice clipping shear male and female, field or water culture experiments. On this basis, the experiment was repeated again in 1982 in Liangping and Canxi. in