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目前,皂素主要是从野生薯蓣的根茎中提取,一般收得率约2%左右。为了满足避孕药物及甾体药物生产对皂素原料的需求,我们摸索了盾叶薯蓣的组织培养,现初步报道这一试验结果。材料和方法取盾叶薯蓣的根茎、幼茎(生长点下5—7厘米)、幼叶(未展开的小叶片)、茎尖(生长点)在无菌箱内,用70%乙醇灭菌3—5分钟,用无菌水洗
At present, saponin is mainly extracted from the roots of wild yam, generally about 2% yield. In order to meet the contraceptives and steroid drug production needs of saponin raw materials, we explored the tissue culture of Dioscorea zingiberensis, the results of this preliminary report is now reported. MATERIALS AND METHODS The rhizomes, young stems (5-7 cm below the growing point), young leaves (undeveloped leaflets) and shoot tips (growing point) were sterilized with 70% ethanol 3-5 minutes, washed with sterile water