论文部分内容阅读
采用聚合酶链式反应(PCR)技术对山东半岛南岸水域的蓝点马鲛(Scomberomorus niphonius)群体(n=20)的mtDNA D-loop序列进行扩增,获得了大小约为500 bp的扩增产物。PCR产物经纯化后进行序列测定,得到了410bp的核苷酸片段(除去引物及部分端部序列)。用Genedoc软件进行排序比较,在这20个个体中,共检测到54个变异位点,包括2个碱基缺失、1个碱基插入、43个转换位点、7个颠换位点及2个转换与颠换同时存在的位点。运用MEGA软件计算出不同个体间的遗传距离,并据此构建了UPGMA和NJ系统树。用DNASP软件计算出的多态位点数(S)为54、核苷酸多样性(P_i)和平均核苷酸差异数(K)分别为0.0271和11.047。研究结果表明,蓝点马鲛的mtDNA D-loop基因个体变异程度较大,适合于群体内及群体间不同个体的遗传多样性分析。
The mtDNA D-loop sequence of Scomberomorus niphonius population (n = 20) in the southern shore of Shandong Peninsula was amplified by polymerase chain reaction (PCR), and the amplification was about 500 bp in size product. The PCR product was purified and sequenced, resulting in a 410 bp nucleotide fragment (primers and partial end sequences removed). A total of 54 mutations were detected in these 20 individuals using the Genedoc software, including 2 base deletions, 1 base insertion, 43 conversion sites, 7 transversion sites and 2 A conversion and transversion exist at the same time. Using MEGA software to calculate the genetic distance between different individuals, and based on which the UPGMA and NJ phylogenetic trees were constructed. The number of polymorphic loci (S) calculated by DNASP software was 54, and the nucleotide diversity (P_i) and mean nucleotide difference (K) were 0.0271 and 11.047, respectively. The results showed that the genetic variation of mtDNA D-loop gene in B. punctata was large, which was suitable for the analysis of genetic diversity among individuals in different populations.