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目的探讨海南黎汉族冠心病(CHD)血管紧张素转换酶(ACE)基因多态性与危险因素的关系。方法采用聚合酶链反应(PCR)方法,检测海南黎族CHD患者150例及正常对照组150例以及汉族CHD患者150例及汉族正常对照组150例的ACE基因D/I多态性,观察DD、DI、II基因型频率,并对所有普通PCR定为DD型的样本进行插入特异性PCR检测,以减少误分型率。结果海南黎族冠心病组DD、DI、II基因的频率分别是24.7%、32.7%、42.6%,D及I等位基因频率分别为41.0%、59.0%。黎族正常对照组DD、DI、II基因型频率分别为14.0%、44.0%、42.0%,D及I等位基因频率分别为36.0%、64.0%,两组之间的DD、DI、II基因频率及D、I等位基因频率均有显著性差异(P<0.05);海南汉族冠心病组DD、DI、II基因的频率分别是23.3%、45.4%、31.3%;D及I等位基因频率分别为46.0%、54.0%。正常对照组DD、DI、II基因型频率分别为13.3%、42.0%、44.7%;D及I等位基因频率分别为34.3%、65.7%,两组之间的DD、DI、II基因频率及D、I等位基因频率均有显著性差异(P<0.05)。且经多元逐步回归分析显示,海南黎汉族冠心病TG水平升高,HDL-C降低。结论血管紧张素转换酶多态性与黎汉族冠心病有显著关联,是海南黎汉族冠心病的主要致病基因,甘油三酯(TG)升高,高密度脂蛋白(HDL-C)降低是海南黎汉冠心病的危险因子。
Objective To investigate the relationship between angiotensin converting enzyme (ACE) gene polymorphism and risk factors in CHD coronary heart disease in Hainan Province. Methods Polymerase chain reaction (PCR) was used to detect ACE gene D / I polymorphism in 150 cases of CHD patients in Hainan Li and 150 cases of normal controls as well as 150 CHD patients in Han nationality and 150 normal Han controls. DD, DI, and II genotype frequencies, and inserted PCR-specific PCR was performed on all samples that had been identified as DD by conventional PCR to reduce the mis-typing rate. Results The frequencies of DD, DI and II genes in CHD patients in Hainan were 24.7%, 32.7% and 42.6%, respectively. The frequencies of D and I alleles were 41.0% and 59.0%, respectively. The frequencies of DD, DI, and II genotypes in Li normal control group were 14.0%, 44.0% and 42.0%, respectively. The frequencies of D and I allele were 36.0% and 64.0% (P <0.05). The frequencies of DD, DI and II genes in Han nationality CHD patients were 23.3%, 45.4% and 31.3%, respectively. The frequency of D and I alleles Respectively 46.0%, 54.0%. The frequencies of DD, DI and II genotypes in normal control group were 13.3%, 42.0% and 44.7%, respectively. The frequencies of D and I allele were 34.3% and 65.7% D, I allele frequencies were significantly different (P <0.05). Multiple stepwise regression analysis showed that TG levels of CHD patients in Hainan Lehan group increased and HDL-C decreased. Conclusions There is a significant association between angiotensin converting enzyme polymorphism and coronary heart disease in Lehan. It is the main causative gene of coronary heart disease in Lehan Han Nationality. The triglyceride (TG) and the decrease of high density lipoprotein (HDL-C) Risk factors of coronary heart disease in Hainan Li Han.