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MicroRNAs(mi RNAs)是一类内源性、长度约22 nt的非编码小RNA,通过转录后调控基因的表达水平、参与生物体的生长与发育等多种生理过程。翘嘴鳜是我国淡水经济鱼类,由于长期人工繁殖忽视亲本选育,导致鳜种质退化,出现生长速度及抗病力下降等问题。本研究中,我们以生长存在显著差异的慢长组和快长组翘嘴鳜肌肉为材料,通过HiSeq 2000深度测序技术和生物信息学分析miRNA转录组,鉴定出433个已知miRNAs并分析miRNAs种子编辑情况。Expdiff方法结合qPCR验证试验,证实了4个表达显著差异的mi RNAs:miR-122、miR-192、miR-451、let-7j-5p。对差异miRNAs进行靶基因预测并通过KEGG Pathway显著性富集分析,发现差异miRNA参与生长、发育、代谢、信号转导等途径,其中Wnt信号通路等在肌肉的生长发育过程起重要作用。本研究为进一步开展miRNA-靶基因的交互作用、了解其对鳜鱼生长的调控机制奠定了基础,并可为鳜鱼分子选育种提供候选基因。
MicroRNAs (miRNAs) are endogenous, non-coding small RNAs of about 22 nt in length and are involved in various physiological processes such as growth and development of organisms through post-transcriptional regulation of gene expression. Culter alburnii is a kind of freshwater economic fish in our country. Due to the long-term artificial breeding, the parental breeding is neglected, resulting in the deterioration of germplasm resources, the growth rate and the decline of disease resistance. In this study, 433 known miRNAs were identified and analyzed by using HiSeq 2000 deep sequencing and bioinformatics analysis of slow-growing and fast-growing Culter albino muscles, Seed editing situation. The Expdiff method combined with qPCR validation assay confirmed four mi RNAs that expressed significant differences: miR-122, miR-192, miR-451 and let-7j-5p. Target genes were predicted by differential miRNAs and significant enrichment analysis by KEGG Pathway. Differential miRNAs were found to be involved in growth, development, metabolism and signal transduction, among which Wnt signaling pathway plays an important role in muscle growth and development. This study laid the foundation for the further interaction between miRNA-target genes and their regulation of the growth of anchovy, and provided candidate genes for molecular breeding of anchovy.