论文部分内容阅读
该研究基于课题组前期发现的“藻戟遂芫”与甘草配伍致毒增毒特点,以肠道及其菌群为研究对象,从肠道屏障功能、运动功能、肠道菌群结构稳态、肠道菌群代谢基因等角度,进一步拓展表征功效相似、基源相近、化学成分类型类同的中药千金子与甘草合用可能导致的毒副作用。结果显示千金子与甘草合用导致肠黏膜损伤标记物血清二胺氧化酶含量升高,并且使肠道菌群组成结构发生异常改变,增加S24_7_ukn,Candidatus arthromitus等内毒素合成、肠道免疫等相关菌属至对照组2倍左右,同时使肠道菌群宏基因组结构发生异常,提高芳香氨基酸降解功能和粘液降解功能相关基因含量,从而可能增强这些基因功能,继而增加肠源尿毒素等毒性物质的产生,引发或加重机体患病风险。该实验以肠道菌群作为研究药物相互作用的重要靶点,并将“药物-肠道菌群-代谢产物-毒性”应用于实验之中,为中药配伍禁忌提供了研究思路。
The research based on the group found earlier “algae” and Licorice flavonoids compatibilized with toxic and toxic characteristics of the gut and its flora as the research object, from the intestinal barrier function, motor function, intestinal flora structure steady state , Gut microbiome metabolic genes and other aspects of angle, to further expand the characterization of similar efficacy, similar to the source, the chemical composition of the same type of Chinese medicine Qianjin and licorice may lead to toxic side effects. The results showed that Qianjin combined with licorice lead to intestinal mucosal injury markers of serum diamine oxidase increased, and make the intestinal flora abnormal changes in the structure, increase S24_7_ukn, Candidatus arthromitus and other endotoxin synthesis, intestinal immune and other related bacteria Belong to the control group about 2 times, at the same time make the gut microbiome anomaly structure, improve the aromatic amino acid degradation and mucus degradation related gene content, which may enhance the function of these genes, and then increase intestinal uremic toxins and other toxic substances Produce, trigger or increase the risk of the body. In this experiment, gut microbiota was used as an important target for the study of drug interactions, and “drug - gut flora - metabolites - toxicity” was applied to the experiment, which provided a research idea for incompatibility of traditional Chinese medicine.