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目的:利用电喷雾质谱技术对新西兰牡荆苷、木犀草素-6-C-葡萄糖-8-C-木糖苷、槲皮素-6-C-葡萄糖苷和二氢槲皮素-6-C-葡萄糖苷4种碳苷黄酮的质谱裂解规律进行研究。方法:用电喷雾多级质谱(ESI-MSn)对该类化合物进行研究,并分别在正离子和负离子扫描模式下对其主要特征碎片离子进行解析。结果:负离子扫描模式下,主要发生糖环裂解,六碳糖碳苷主要产生脱去m/z 120,90的碎片峰,五碳糖碳苷主要产生脱去m/z 90,60的碎片峰,双糖碳苷主要产生连续脱去m/z120,90,60的碎片峰;正离子扫描模式下,主要发生糖羟基连续脱水,六碳糖碳苷产生脱去m/z 120的碎片峰,五碳糖碳苷产生脱去m/z 60的碎片峰。碳苷黄酮的负离子扫描模式比正离子扫描模式的质谱特征更明显。结论:分析了这4种碳苷黄酮的电喷雾多级质谱裂解规律,探讨了碳苷黄酮,特别是双糖碳苷黄酮质谱裂解规律,为碳苷黄酮的快速鉴别提供了参考。
OBJECTIVE: To determine the effects of vitexin, luteolin-6-C-glucose-8-C-xyloside, quercetin-6-C-glucoside and dihydroquercetin-6-C - glucoside four kinds of carbohydrate flavonoids were studied by mass spectrometry. Methods: The compounds were studied by electrospray ionization mass spectrometry (ESI-MSn) and their major characteristic fragment ions were analyzed in positive and negative ion scanning mode respectively. Results: In negative ion scanning mode, cleavage mainly occurred in the ring of sugar cane, and the six carbon-carbon glycosides mainly produced fragments of m / z 120 and 90. The five-carbon glycosides mainly produced fragments of m / z 90 and 60 , The double sugar carbon glycosides mainly produce fragment peaks m / z120, 90 and 60 continuously. In the positive ion scanning mode, the continuous dehydration of sugar and hydroxyl groups occurs, and the fragment peak of m / z 120 is removed by the six- The five-carbon glycosidoside produced fragment peaks off m / z 60. The negative ion scan mode of carbon glycoside flavonoids is more obvious than that of positive scan mode. CONCLUSION: The four kinds of carbo-glycosides flavonoids were analyzed by electrospray ionization mass spectrometry (GC-MS). The cleavage patterns of carbo-glycosides flavonoids, especially bis-carbon glycoside flavonoids, were explored, which provided a reference for the rapid identification of glycosides.