论文部分内容阅读
目的 :探讨检测肝硬化患者血清、腹水中一氧化氮 (NO)浓度在肝硬化诊断及治疗中的意义。方法 :用硝酸盐还原酶法测定了肝硬化患者血清 (n =4 0 )和腹水 (n =15)、急性肝炎患者血清 (n =50 )、正常对照组 (n =50 )血清中NO浓度 ,结果 :发现肝硬化患者血清NO浓度较正常对照组及急性肝炎组显著升高 (P <0 .0 1) ,肝硬化患者中有腹水者血清中NO浓度较无腹水者升高更为显著 (P <0 .0 1) ,血清中NO的浓度随Child_Pugh分级增高而升高 (P <0 .0 5) ,肝硬化患者血清中的NO浓度与其腹水中NO浓度呈正相关 (r =0 .6 4 7,P <0 .0 5) ,而急性肝炎患者血清中NO浓度虽有升高 ,但与正常对照组比较无显著性差异 (P >0 .0 5) ,结论 :肝硬化时 ,NO合成增加 ,肝功能的损害和腹水的产生与NO的浓度密切相关 ,检测肝硬化患者血清及腹水中NO浓度 ,对临床判断肝硬化病情严重程度和观察疗效具有一定的帮助。
Objective: To investigate the significance of detection of nitric oxide (NO) in serum and ascites of cirrhotic patients in the diagnosis and treatment of liver cirrhosis. Methods: Serum NO (n = 40), ascites (n = 15), acute hepatitis (n = 50) and normal control group (n = 50) were measured by nitrate reductase method in patients with cirrhosis. Results: The serum concentration of NO in patients with cirrhosis was significantly higher than that in normal control group and acute hepatitis group (P <0.01). The serum NO in patients with cirrhosis was significantly higher than that without ascites (P <0.01). The concentration of NO in serum increased with the increase of Child_Pugh classification (P <0.05). The concentration of NO in serum of patients with cirrhosis was positively correlated with the concentration of NO in ascites (P <0.01). (P 0. 05). However, the concentration of NO in the serum of patients with acute hepatitis increased, but there was no significant difference compared with the normal control group (P0.05) .Conclusion: In cirrhotic patients, The increase of NO synthesis, the damage of liver function and the production of ascites are closely related to the concentration of NO. Detecting the concentration of NO in serum and ascites of patients with cirrhosis of liver cirrhosis is helpful to judge the severity of cirrhosis and observe the curative effect.