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1 材料和方法 用 3 H- Td R掺入法检测了 P物质 (SP)对体外培养的大鼠脾细胞增殖的影响 ,用 CTLL细胞株结合 3 H- Td R掺入法检测了脾细胞分泌 IL - 2的变化 ,用放射免疫测定的方法测定了脾细胞培养上清液中IL - 6和 TNF的变化 ,并用流式细胞仪计数了脾细胞中 T细胞表型表达
1 Materials and methods 3 H-Td R incorporation assay of substance P (SP) on the proliferation of rat spleen cells cultured in vitro, with CTLL cell lines combined with 3 H-Td R splenocyte secretion assay was measured The changes of IL - 6 and TNF in culture supernatants of splenocytes were measured by radioimmunoassay. The expression of T cell phenotype in spleen cells was counted by flow cytometry