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在乙肝病毒高分辨率免疫识别的基础上，以Ｐｒｅ－Ｓ２蛋白的优势Ｂ细胞表位Ｂ１、Ｂ２为基础，引入不同的Ｔｈ细胞表位Ｔｈ１、Ｔｈ２，结构参照ＭＡＰ设计，采用固相合成法，合成了ＭＡＰ１和ＭＡＰ２。产物经毛细管电泳分析和氨基酸组分鉴定。本研究为不同表位的重新组合构建新型多肽抗原及疫苗提供了线索
On the basis of high-resolution immunological recognition of hepatitis B virus, different Th cell epitopes Th1 and Th2 were introduced based on the preferential B-cell epitopes B1 and B2 of Pre-S2 protein. The structural reference MAP design was adopted and the solid-phase synthesis method , Synthesized MAP1 and MAP2. The product was analyzed by capillary electrophoresis and amino acid component identification. This study provides clues for recombination of different epitopes to construct novel polypeptide antigens and vaccines