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目的:验证转内皮抑素基因抑制高转移性黑色素瘤细胞的转移能力。方法:将pcDNA3.1-Endo真核表达载体转染小鼠黑色素瘤高转移细胞株B16F10,运用RT-PCR和W estern-b lot验证Endo的表达后,进行瘤细胞的粘附实验、体外侵袭和运动实验以及C57BL/6小鼠的皮下种植瘤和肺转移瘤试验,并进一步统计分析。结果:内皮抑素基因能明显抑制B16F10黑色素瘤细胞的粘附、体外侵袭和运动、体内成瘤以及肺转移能力,其中粘附抑制率36.4%,体外侵袭抑制率48.4%,细胞运动功能抑制率52.1%,肺转移抑制率为67.3%。结论:转内皮抑素基因能明显抑制黑色素瘤细胞的高转移能力。
Objective: To demonstrate that endostatin gene inhibits the metastatic ability of highly metastatic melanoma cells. Methods: The pcDNA3.1-Endo eukaryotic expression vector was transfected into mouse melanoma cell line B16F10. The expression of Endo was verified by RT-PCR and Western-blot, and the adhesion assay of tumor cells was performed in vitro And exercise experiments and C57BL / 6 mice subcutaneously implanted tumor and lung metastases test, and further statistical analysis. Results: Endostatin gene could significantly inhibit the adhesion, invasion and movement of B16F10 melanoma cells in vitro, tumorigenesis and lung metastasis in vivo. The adhesion inhibition rate was 36.4%, the in vitro invasion inhibition rate was 48.4%, the inhibition rate of motor function 52.1%, pulmonary metastasis rate was 67.3%. Conclusion: Endostatin gene can significantly inhibit the high metastatic capacity of melanoma cells.