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目的探讨白介素-10(IL-10)基因-627位点多态性与昆明地区儿童哮喘的相关性。方法采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)分析方法,检测50例哮喘儿童和36例健康儿童IL-10基因-627位点基因型,比较两组IL-10基因-627位点的基因型和等位基因分布频率。结果哮喘组和健康组3种基因型CC、CA、AA分别为:12.0%、48.0%、40.0%;8.3%、50.0%、41.7%。两组基因型分布差异无显著性(P>0.05)。哮喘组和健康组C和A的等位基因分布频率分别为:36.0%、64.0%;31.9%、68.1%。两组等位基因分布频率差异无显著性(P>0.05)。结论本研究表明IL-10基因-627位点多态性可能与昆明地区儿童哮喘易感性无关。
Objective To investigate the association of interleukin-10 (IL-10) -627 polymorphism with childhood asthma in Kunming. Methods Genotypes of -627 loci in IL-10 gene of 50 asthmatic children and 36 healthy children were detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The IL-10 gene -627 loci genotype and allele distribution frequency. Results The genotypes of CC, CA and AA in asthma group and healthy group were 12.0%, 48.0%, 40.0%, 8.3%, 50.0% and 41.7%, respectively. There was no significant difference in genotype distribution between the two groups (P> 0.05). The allele distribution frequencies of C and A in asthma group and healthy group were 36.0%, 64.0%, 31.9% and 68.1% respectively. There was no significant difference in allele frequencies between the two groups (P> 0.05). Conclusion This study shows that IL-10 gene-627 polymorphism may be unrelated to asthma susceptibility in children in Kunming.