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目的了解铅接触及铅中毒工人外周血中T细胞信号传导分子CD3ζ基因表达水平,为铅接触和铅中毒工人免疫系统损害的机制提供资料。方法利用SYBR GreenⅠ荧光定量PCR分别检测10例铅接触工人、6例铅中毒工人和20例正常人外周血单个核细胞的CD3ζ基因表达情况,以β2微球蛋白基因(β2M)作为内参,根据相对定量公式(2-△Ct)计算铅接触、铅中毒工人与正常人CD3ζ基因表达水平差异。结果正常人、铅接触和铅中毒工人外周血CD3ζ基因表达水平分别为(3.01±2.11)、(11.4±13.3)和(2.42±3.01)。与正常人相比,铅接触工人外周血CD3ζ基因表达上升(P<0.05)。与铅接触工人相比,铅中毒工人CD3ζ基因表达下降(P<0.05)。结论铅接触及铅中毒工人中外周血T细胞CD3ζ基因表达异常,提示机体接触铅后T细胞活化过程存在障碍。
Objective To understand the expression of T cell signaling molecule CD3ζ in peripheral blood of lead exposed and lead poisoning workers and provide information for the mechanism of lead exposure and the damage to the immune system of lead poisoning workers. Methods The expression of CD3ζ gene in peripheral blood mononuclear cells of 10 lead exposed workers, 6 lead poisoned workers and 20 normal controls were detected by SYBR Green Ⅰ fluorescent quantitative PCR. Β2 microglobulin gene (β2M) Quantitative formula (2- △ Ct) to calculate lead exposure, lead poisoning workers and normal subjects CD3ζ gene expression levels. Results The levels of CD3ζ in peripheral blood of workers exposed to lead and lead poisoning were (3.01 ± 2.11), (11.4 ± 13.3) and (2.42 ± 3.01), respectively. Compared with normal controls, the expression of CD3ζ in peripheral blood of lead exposed workers increased (P <0.05). Compared with lead exposure workers, lead poisoning workers CD3ζ gene expression decreased (P <0.05). Conclusion Abnormal expression of CD3ζ gene in peripheral blood T lymphocytes from lead exposure and lead poisoning workers suggests that there is an obstacle to the activation of T cells after exposure to lead.