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目的:对中国HIV-1病毒分离株p6区进行遗传多态性分析,了解p6区突变模式,为进一步研究p6区与HIV-1耐药性的相互关系提供有价值资料。方法:采集中国不同地区HIV/AIDS患者的抗凝全血,分离血浆,采用套式RT-PCR方法扩增全长p6区,对其测序并应用生物信息学软件进行遗传多态性分析。结果:HIV-1 p6区特定区域和位点发生高频率突变,包括点突变、插入和缺失突变。点突变位点主要集中在17,19,25,39,42,47等位点,发生在PTAP结构域的插入突变多为PTAP加倍导致,其发生频率高达11%,而其突变形式多为PTAPAPS和PTAPVPT。结论:中国HIV-1病毒分离株p6区具有高度的遗传多态性。
OBJECTIVE: To analyze the genetic polymorphism of p6 region of HIV-1 isolates in China, and to understand the mutation pattern of p6 region, so as to provide valuable data for further study on the relationship between p6 region and HIV-1 drug resistance. Methods: Anticoagulant whole blood was collected from HIV / AIDS patients in different regions of China. Plasma was collected. The full length p6 region was amplified by nested RT-PCR. The full length p6 region was sequenced and bioinformatics software was used to analyze the genetic polymorphism. RESULTS: High-frequency mutations occurred in specific regions and sites of HIV-1 p6 region, including point mutations, insertions and deletions. Point mutation sites are mainly concentrated in the 17,19,25,39,42,47 and other sites, occurred in the PTAP domain insertion mutation more than doubled PTAP, its frequency up to 11%, and its mutation is mostly PTAPAPS And PTAPVPT. Conclusion: The p6 region of HIV-1 isolate in China has a high degree of genetic polymorphism.