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目的研究分化型甲状腺癌(DTC)患者内源性甲状腺球蛋白抗体(TgAb)是否干扰DTC患者血清甲状腺球蛋白(Tg)的测定,分析这种干扰作用是否与TgAb浓度呈剂量依赖性。方法以DTC随访患者作为研究对象。选择Tg>10μg/L、TgAb阴性作为Tg阳性血清,TgAb>115IU/mL、Tg阴性作为TgAb干扰血清。以恒定量的Tg阳性血清与容积逐渐增加的TgAb干扰血清混合,室温过夜,电化学发光法(ICMA)测定各管Tg值。以TgAb浓度为横坐标、Tg测定值为纵坐标绘图。结果在8例DTC患者的Tg阳性血清中加入3例DTC患者内源性TgAb干扰血清,共获得8条TgAb-Tg曲线。加入TgAb阳性血清后,Tg实测值均降低,其中3例患者的Tg测定值降低幅度与TgAb浓度相关,5例不相关。结论采用免疫分析法测定血清Tg值,内源性TgAb降低了Tg测定值。但只有部分DTC患者Tg测定值降低幅度与TgAb浓度呈剂量依赖性。
Objective To investigate whether endogenous thyroglobulin antibody (TgAb) in patients with differentiated thyroid cancer (DTC) interfered with the determination of serum thyroglobulin (Tg) in patients with DTC and to determine whether this interference was dose-dependent with the concentration of TgAb. Methods DTC follow-up patients as the research object. Select Tg> 10μg / L, TgAb negative as Tg positive serum, TgAb> 115IU / mL, Tg negative as TgAb interference serum. The serum was mixed with a constant amount of Tg positive serum and a gradually increasing volume of TgAb, overnight at room temperature, and the Tg of each tube was determined by the electrochemiluminescence method (ICMA). With TgAb concentration as abscissa and Tg as ordinate. Results The endogenous TgAb interfering sera of 3 DTC patients were added to the Tg positive sera of 8 patients with DTC. Eight TgAb-Tg curves were obtained. The addition of TgAb-positive serum, Tg measured values were reduced, of which 3 cases of patients with Tg measured value and TgAb concentration related to 5 cases were not related. Conclusion The serum Tg was measured by immunoassay, and the endogenous TgAb decreased the value of Tg. However, only a portion of DTC patients had a dose-dependent decrease in Tg measured with TgAb.