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目的:制备负载胰岛素壳聚糖-醋酸乙烯酯共聚物纳米粒并研究其性能。方法:用自由基聚合法合成壳聚糖-醋酸乙烯酯共聚物,该聚合物在水中形成具有疏水核心、亲水表面的纳米粒。采用正交设计试验研究了投料比、引发剂浓度和反应时间对纳米粒粒径的影响。结果:对纳米粒进行了热重分析和红外表征。测定纳米粒的形态、粒径和表面电位(Zeta电位),以胰岛素为模型药物,研究纳米粒的包封和释药性能。结论:纳米粒呈球形,粒径均匀,表面荷正电。胰岛素的包封率可达90%以上。pH6.8的磷酸盐缓冲液中胰岛素释放较慢。结论:该纳米制剂具有较好的物理性能和体外缓释特性。
Objective: To prepare insulin-loaded chitosan-vinyl acetate copolymer nanoparticles and study its properties. Methods: Chitosan-vinyl acetate copolymer was synthesized by free radical polymerization. The polymer formed nanoparticles with hydrophobic core and hydrophilic surface in water. Orthogonal design experiments were carried out to study the effect of feed ratio, initiator concentration and reaction time on the particle size. Results: Thermal gravimetric analysis and infrared characterization of the nanoparticles. The morphology, particle size and surface potential (Zeta potential) of the nanoparticles were determined. Insulin was used as a model drug to study the encapsulation and drug release properties of the nanoparticles. Conclusion: The nanoparticles are spherical, uniform in size and positively charged on the surface. Insulin encapsulation efficiency up to 90%. Insulin release is slower in pH 6.8 phosphate buffer. Conclusion: The nano-preparation has good physical properties and in vitro release characteristics.