论文部分内容阅读
HEL细胞是一株人红白血病细胞株,其中成年型β-珠蛋白基因不能表达。我们以往的试验证明,羟基脲诱导以后,能使HEL细胞内成年型β-珠蛋白基因表达。本文以此为模型,探索了β-珠蛋白基因在HEL细胞内诱导表达的分子机制。结果表明,羟基脲诱导之后,与人β-珠蛋白基因5’远侧端DNaseⅠ超敏感点2核心DNA序列以及近侧端启动子DNA序列相结合的GATA-1蛋白因子量明显增多,而GATA-2蛋白因子量明显减少。结果显示,GATA蛋白家族各成员在HEL细胞分化及珠蛋白基因表达的过程中扮演着不同的角色。推测GATA-1可能有促进β-珠蛋白基因的表达,使HEL细胞趋向终末分化的作用,GATA-2则可能与胚胎型珠蛋白基因表达有关,并有抑制红细胞向终末分化的功能。
HEL cells are a human erythroleukemia cell line in which the adult β-globin gene cannot be expressed. Our previous experiments have demonstrated that hydroxyurea induces the expression of the mature β-globin gene in HEL cells. Using this model as a model, we explored the molecular mechanism of the induction of β-globin gene expression in HEL cells. The results showed that after the induction of hydroxyurea, the amount of GATA-1 protein factor that binds to the DNA sequence of the DNase I hypersensitive 2 core site and the proximal end promoter of the human β-globin gene was significantly increased. The amount of -2 protein factor was significantly reduced. The results showed that members of the GATA protein family play different roles in HEL cell differentiation and globin gene expression. It is speculated that GATA-1 may promote the expression of β-globin gene and make HEL cells terminally differentiated. GATA-2 may be related to the expression of embryonic globin gene and have the function of inhibiting the terminal differentiation of erythrocytes.