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AIM:TO study the effect of staurosporine (ST) on the cellcycle of human gastric cancer cell lines MGC803 andSGC7901.METHODS:Cell proliferation was evaluated by trypan bluedye exclusion method.Apoptotic morphology was observedunder a transmission electron microscope.Changes of cellcycle and apoptotic peaks of cells were determined by flowcytometry.Expression of p21~(WAFl)gene was examined usingimmunohistochemistry and RT-PCR.RESULTS:The growth of MGC803 and SGC7901 cells wasinhibited by ST.The inhibitory concentrations against 50%cells (IC_(50)) at 24 h and 48 h were 54 ng/ml and 23 ng/ml forMGC803,and 61 ng/ml and 37 ng/ml for SGC7901.Typicalapoptotic bodies and apoptotic peaks were observed 24 hafter cells were treated wth ST at a concentration of 200ng/ml.The percentage of cells at G_0/G_1 phase was decreasedand that of cells at G_2/M was increased significantly in thegroup treated wth ST at the concentrations of 40 ng/ml,60 ng/ml,100 ng/ml for 24 h,compared with the controlgroup (P<0.01).The expression levels of p21~(WAFl)gene inboth MGC803 and SGC7901 cells were markedly up-regulatedafter treatment with ST.CONCLUSION:ST can cause arrest of gastric cancer cellsat G_2/M phase,which may be one of the mechanisms thatinhibit cell proliferation and cause apoptosis in these cells.Effect of ST on cells at G_2/M phase may be attributed to theup-regulattion of p21~(WAFl) gene.
AIM: TO study the effect of staurosporine (ST) on the cell cycle of human gastric cancer cell lines MGC803 and SGC7901.METHODS: Cell proliferation was evaluated by trypan bluedye exclusion method. Apoptotic morphology was observedunder a transmission electron microscope. Cells of cell cycle and apoptotic peaks of cells were determined by flow cytometry. Expression of p21 WAF1 gene was examined using immunohistochemistry and RT-PCR. RESULTS: The growth of MGC803 and SGC7901 cells was inhibited by ST.The inhibitory concentrations against 50% cells (IC 50) 24 h and 48 h were 54 ng / ml and 23 ng / ml forMGC803, and 61 ng / ml and 37 ng / ml for SGC7901.Typicalapoptotic bodies and apoptotic peaks were observed 24 hafter cells were treated wth ST at a concentration of 200 ng / ml. The percentage of cells at G_0 / G_1 phase was decreased and that of cells at G_2 / M was increased significantly in the group treated wth ST at the concentrations of 40 ng / ml, 60 ng / ml, 100 ng / ml for 24 h, compared with the control group (P < 0.01) .The expression levels of p21 WAF1 gene inboth MGC803 and SGC7901 cells were markedly up-regulated after treatment with ST.CONCLUSION: ST can cause arrest of gastric cancer cellsat G_2 / M phase, which may be one of the mechanisms that inhibit cell proliferation and cause apoptosis in these cells. Effect of ST on cells at G_2 / M phase may be attributed to the up-regulattion of p21 ~ (WAF1) gene.