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Both purified methane monooxygenase enzyme system and restingwhole-cell suspensions of Methylomonas sp.strain GYJ-3 were used as biocata-lysts for methanol biosynthesis from methane.The productivity which cat-alyzed by highly purified enzyme system was 65～70 μmol/mg in the presenceof dioxygen and NADH during 2h reaction course.Samll amounts of methanol(approximately 0.24 mmol/L)accmulated when washed cell suspensions ofMethylomonas sp.strain GYJ-3 were incubated with CH_4+O_2 mixture in coni-cal flasks.The optimal temperature,pH,buffer concentration and reaction timewere reported.The results presented here provide a direct evidence for methanemetablism to CO_2 via methanol in whole-cell of the bacterium.