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目的:对人无精子症相关基因进行聚类分析。方法:应用包含人cDNA微矩阵芯片对人正常睾丸及无精子症睾丸组织中的差异表达基因进行研究。芯片包含的基因按照功能分为原癌基因和抑癌基因、离子通道和运输蛋白、细胞周期蛋白类、外压反应蛋白、细胞骨架和运动、细胞凋亡相关蛋白、DNA合成修复重组蛋白、DNA结合转录和转录因子、细胞受体、免疫相关、代谢蛋白、翻译合成、发育相关、细胞信号和传递蛋白以及其他(新发现及功能不清)15项,对获得的差异基因进行聚类分析。结果:共得到158个差异基因,新近发现及功能不清的部分基因与蛋白翻译合成(51、19个)、代谢相关(18个)、细胞信号和传递(15个)、原癌基因和抑癌基因(13个)等在杂交中表现出优于其他功能种类的基因改变。结论:对上述基因的相关研究有助于加深对精子生成障碍机制的认识。
Objective: To cluster the related genes of human azoospermia. Methods: Human cDNA microarray was used to study differentially expressed genes in human testis and testis of azoospermia. Chips contain genes that are classified into proto-oncogenes and tumor suppressor genes, ion channels and transport proteins, cyclins, PPARs, cytoskeleton and motility, apoptosis-related proteins, DNA synthesis and repair of recombinant proteins, DNA Fifteen combinations of transcriptional and transcriptional factors, cellular receptors, immune-related, metabolic proteins, translational synthesis, developmental relationships, cellular signaling and transfer proteins, and other (newly discovered and dysfunctional) genes were clustered using the cluster analysis. RESULTS: A total of 158 differentially expressed genes were found, some of the newly discovered and uncleaved genes were associated with protein translation (51,19), metabolism (18), cell signaling and transmission (15), proto-oncogene Oncogenes (13) and others showed better genetic modification than other functional types in hybridization. CONCLUSIONS: The relevant studies on the above genes help to deepen the understanding of the mechanism of spermatogenesis disorder.