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目的:探讨细胞凋亡与肺缺血再灌注损伤的关系以及红花注射液的干预作用及其机制。方法:健康日本大耳白兔36只,随机分为对照组(C组)、缺血再灌注组(IR组)和红花干预组(SI组)。复制在体肺缺血再灌注损伤模型。对比观察各组肺湿干重比(W/D)、肺泡损伤数定量评价指标(IQA);采用原位缺口末端标记(TUNEL)法观测肺组织细胞凋亡指数,原位杂交技术检测肺组织细胞caspase-3基因的表达。结果:IR组W/D、IQA、肺组织细胞凋亡指数及caspase-3mRNA表达均显著高于C组(P<0.01)。SI组上述各指标明显低于IR组(P<0.01或P<0.05)。肺组织细胞凋亡指数与W/D、IQA及caspase-3mRNA均呈显著正相关(r分别=0.920,0.925,0.927;P均<0.01)。结论:肺组织细胞凋亡参与了肺缺血再灌注损伤的发生,红花注射液可能通过下调caspase-3的表达抑制肺组织细胞凋亡,从而减轻肺缺血再灌注损伤。
Objective: To investigate the relationship between cell apoptosis and lung ischemia-reperfusion injury, and the intervention and mechanism of Honghua injection. METHODS: Thirty-six healthy Japanese white rabbits were randomly divided into control group (C group), ischemia-reperfusion group (IR group) and safflower intervention group (SI group). Replicate models of lung ischemia reperfusion injury. Observe the quantitative index of lung wet/dry weight ratio (W/D) and alveolar damage (IQA) in each group; observe the apoptotic index of lung tissue by in situ nick end labeling (TUNEL) method; and detect lung tissue by in situ hybridization Cell caspase-3 gene expression. Results: W/D, IQA, apoptosis index and caspase-3 mRNA expression in IR group were significantly higher than those in C group (P<0.01). The above indicators in the SI group were significantly lower than those in the IR group (P<0.01 or P<0.05). The apoptotic index of lung tissue was positively correlated with W/D, IQA and caspase-3 mRNA (r=0.920, 0.925, 0.927; P<0.01). Conclusion: Apoptosis of lung tissue is involved in the occurrence of lung ischemia-reperfusion injury. Honghua injection may inhibit the apoptosis of lung tissue by down-regulating the expression of caspase-3, thus alleviating lung ischemia-reperfusion injury.