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目的:在体外建立大鼠耳蜗螺旋神经节神经元培养模型,并提高其产出率及细胞活性。方法:将出生后5天的大鼠耳蜗螺旋神经节神经元在体外培养3～5天,用神经丝蛋白(Neurofilament protein,NFP)单克隆抗体进行免疫组化染色。结果:显示耳蜗螺旋神经节神经元在体外无血清培养条件下,可以存活并进行正常分化。结论:耳蜗螺旋神经节神经元在体外有稳定的可塑性及轴突再生修复能力。
OBJECTIVE: To establish a culture model of rat cochlear spiral ganglion neurons in vitro and to improve its yield and cell viability. Methods: Rat cochlear spiral ganglion neurons were cultured in vitro for 5 days and 5 days after birth, and immunohistochemically stained with Neurofilament protein (NFP) monoclonal antibody. Results: The cochlear spiral ganglion neurons showed survival and normal differentiation in vitro without serum culture. Conclusion: Cochlear spiral ganglion neurons have stable plasticity and axon regeneration and repair ability in vitro.