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Mesenchymal stem cells have been previously shown to exert an immunomodulatory function.The present study sought to investigate the effects of multipotential human adipose tissue-derived mesenchymal stem cells(hAdMSCs) on disease progression and cytokine expression in Lewis rats with experimental autoimmune encephalomyelitis(EAE) induced by myelin basic protein.The duration of EAE paralysis in the group treated on day 7 postimmunization with 5 × 106 hAdMSCs was significantly reduced compared with the vehicle-treated controls and the 1 × 106 hAdMSC-treated group.The duration of EAE paralysis in the groups treated with 5 × 106 hAdMSCs on both day 1 and day 7 postimmunization was significantly reduced compared with the vehicle-treated controls and the groups treated with 5 × 106 hAdMSCs on both day 7 and day 10 postimmunization.The mRNA expression of interleukin-10 and indoleamine 2,3-dioxygenase was significantly decreased in the hAdMSC-treated group compared with the vehicle-treated group.These findings suggest that the ameliorative effects of hAdMSCs on EAE symptoms operate in a dose-and time-dependent manner and can be mediated in part by the ample production of anti-inflammatory cytokines.
Mesenchymal stem cells have been previously shown to exert an immunomodulatory function. The present study sought to investigate the effects of multipotential human adipose tissue-derived mesenchymal stem cells (hAdMSCs) on disease progression and cytokine expression in Lewis rats with experimental autoimmune encephalomyelitis (EAE) induced by myelin basic protein.The duration of EAE paralysis in the group treated on day 7 postimmunization with 5 × 106 hAdMSCs was significantly reduced compared with the vehicle-treated controls and the 1 × 106 hAdMSC-treated group. The duration of EAE paralysis in the groups treated with 5 × 106 hAdMSCs on both day 1 and day 7 postimmunization was significantly reduced compared with the vehicle-treated controls and the groups treated with 5 × 106 hAdMSCs on both day 7 and day 10 postimmunization.The mRNA expression of interleukin- 10 and indoleamine 2,3-dioxygenase was significantly decreased in the hAdMSC-treated group compared with the vehicle-treated group. The findings suggest that the ameliorative effects of hAdMSCs on EAE symptoms operate in a dose-and time-dependent manner and can be mediated in part by the ample production of anti-inflammatory cytokines.