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目的了解吉林省2013年柯萨奇病毒A组16型(CVA16)流行特征、种系进化及基因分型。方法对吉林省2013年分离获得的9株CVA16阳性毒株的VP1编码区核苷酸进行扩增及序列测定,使用Mega 4.0和Bioedit软件进行序列分析。结果 9株CVA16流行株中,1株为B1a基因亚型,与3株B1a代表株相比,其核苷酸和氨基酸的同源性分别为95.6%~96.8%和99.6%;8株为B1b基因亚型,与3株B1b代表株相比较,其核苷酸和氨基酸的同源性分别为94.1%~96.1%和98.3%~100%。2013年流行株与代表株相比共发生了9处氨基酸位点变异,其中8株B1b亚型中有6株在第23位氨基酸位点发生了亮氨酸→蛋氨酸的变异。结论 B1b是引起吉林省2013年CVA16流行的主要基因亚型,并且有大部分CVA16流行株在第23氨基酸位点发生了共同变异。
Objective To understand the epidemiology, germline evolution and genotyping of Coxsackievirus A group 16 (CVA16) in Jilin province in 2013. Methods The nucleotides of the VP1 coding region of nine CVA16 positive isolates isolated from Jilin Province in 2013 were amplified and sequenced. The sequences were analyzed by Mega 4.0 and Bioedit software. Results Among nine strains of CVA16, one was B1a subtype. The nucleotide and amino acid identities of the strains were 95.6% -96.8% and 99.6% Compared with the three representative strains of B1b, their nucleotide and amino acid homologies were 94.1% -96.1% and 98.3% -100%, respectively. A total of 9 amino acid site mutations were observed in the strains of the epidemic strains in 2013 compared with the representative strains. Among them, 6 of the 8 B1b subtypes had a leucine → methionine mutation at the 23rd amino acid site. Conclusion B1b is the major gene subtype causing the epidemic of CVA16 in Jilin Province in 2013, and most of the strains of CVA16 share common mutations at the 23rd amino acid site.