论文部分内容阅读
目的 探讨肝纤维化发生后MMP 2的基因表达与酶活性的变化及复方“86 1”的影响。方法 4 5只雌性Wistar大鼠随机分为假手术组、胆管阻塞性肝纤维化模型组和复方“86 1”治疗组。术后第 7天开始治疗 ,4 9d后 ,采用半定量RT PCR法检测肝组织中MMP 2mRNA ,应用酶谱法检测肝组织中MMP 2酶活性。结果 模型组肝组织中MMP 2mRNA的表达比正常组高 ,差异有非常显著性(P <0 .0 0 1) ;而中药复方“86 1”治疗组肝组织中MMP 2mRNA的表达 ,与正常组比较差异无显著性 ,比模型组低 ,差异有非常显著性 (P <0 .0 0 2 )。正常组肝组织中未测到MMP 2酶活性 ;模型组肝组织中显示出一定MMP 2酶活性 ;而中药复方“86 1”治疗组肝组织中MMP 2酶活性比模型组显著升高 (P <0 .0 1)。结论 中药复方“86 1”能抑制MMP 2的基因表达 ,但又因可能解除MMP 2的抑制因素而提高其活性 ,以降解沉积的纤维性胶原而参与肝纤维化的逆转过程。
Objective To investigate the changes of gene expression and enzyme activity of MMP 2 after liver fibrosis and the effect of compound “86 1”. Methods Forty-five female Wistar rats were randomly divided into sham operation group, bile duct obstructive hepatic fibrosis model group and compound “86 1” treatment group. The treatment was started on the 7th day after the operation. After 4d, the MMP 2 mRNA in the liver tissue was detected by semi-quantitative RT PCR. The activity of MMP 2 in the liver tissue was detected by zymography. Results The expression of MMP 2 mRNA in liver tissue was higher in the model group than in the normal group (P < 0.01), while the expression of MMP 2 mRNA in the liver tissue of the Chinese herbal compound “86 1” treatment group was normal. There was no significant difference between the two groups, which was lower than the model group. The difference was significant (P < 0.05). The MMP 2 enzyme activity was not detected in the liver tissue of the normal group; the MMP 2 enzyme activity was shown in the liver tissue of the model group; and the MMP 2 enzyme activity in the liver tissue of the Chinese herbal compound “86 1” treatment group was significantly higher than that of the model group (P <0. 0 1). Conclusion Chinese herbal formula “86 1” can inhibit the expression of MMP 2 gene, but it may also increase the activity of MMP 2 because it may release inhibitory factors. It can degrade deposited fibrous collagen and participate in the reversal of hepatic fibrosis.