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目的探讨HPV免疫组化和原位核酸杂交技术在女阴尖锐湿疣和假性湿疣诊断中的价值。方法通过免疫组化和原位核酸杂交技术 ,回顾性分析 2 33例女阴尖锐湿疣、假性湿疣以及湿疣样病变中HPV Ag和HPV6/ 1 1 的检出率并与病理组织学诊断作对比分析。结果 193例尖锐湿疣HPV Ag阳性 10 2例 (占 5 2 85 % ) ,HPV6/ 1 1 阳性 187例 (占 96 89% ) ,两种检测法阳性率的差异具有极显著意义 (P <0 0 1)。 2 0例湿疣样病变HPV Ag阳性 3例 (占 15 % ) ,HPV6/ 1 1 阳性 7例 (占 35 % )。 2 0例假性湿疣HPV Ag和HPV6/ 1 1 均阴性。病理上尖锐湿疣可见于诊断性挖空细胞、角化不全、棘层肥厚、基底细胞增生和上皮脚延长融合 ;而假性湿疣无诊断性挖空细胞 ,无明显棘层肥厚和基底细胞增生。结论HPV原位核酸杂交技术优于免疫组化技术 ,HPV6/ 1 1 的检测有助于鉴别尖锐湿疣和假性湿疣以及湿疣样病变。
Objective To investigate the value of HPV immunohistochemistry and in situ hybridization in the diagnosis of genital warts and pseudohypoglossus. Methods The positive rates of HPV Ag and HPV6 / 1 1 in 2 33 cases of condyloma acuminata, pseudotumor condyloma and condyloma acuminata were retrospectively analyzed by immunohistochemistry and in situ hybridization in situ hybridization. The detection rate was compared with that of histopathological diagnosis analysis. Results 193 cases of condyloma acuminata HPV Ag positive 10 2 cases (5285%), HPV6 / 1 1 positive 187 cases (96 89%), the difference between the two test positive rate was extremely significant (P <0 0 1). Twenty cases of genital warts were HPV positive in 3 cases (15%) and HPV6 / 1 1 positive in 7 cases (35%). 20 cases of false condyloma HPV Ag and HPV6 / 1 1 were negative. Pathologically, condyloma acuminatum can be found in diagnostic koilocyte, keratosis, acanthosis, basal cell hyperplasia and prolonged fusion of epithelial feet; while pseudohypoglossus without diagnostic koilocyte, there is no obvious acanthosis and basal cell hyperplasia. Conclusion HPV in situ hybridization is superior to immunohistochemistry. The detection of HPV6 / 1 1 is helpful to distinguish condyloma acuminatum, pseudokillis and genital warts.