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本研究采用细胞介导微量细胞毒测定法检测139例恶性肿瘤患者和355例健康个体外周血自然杀伤细胞(NK细胞)对用~(125)IUdR标记的K562靶细胞的细胞毒直接杀伤效应。结果表明,肿瘤患者NK细胞活性显著低于健康个体,其中,肝胆肿瘤和晚期肿瘤患者NK活性降低尤为显著,临床治愈者NK活性恢复到正常人水平。不同临床阶段肿瘤患者经过肿瘤手术切除以及药物加放射线治疗好转者NK活性均显著升高。健康个体随着年龄增长NK活性下降,说明非特异免疫监视能力减弱与肿瘤的发生、发展关系密切。
In this study, cell-mediated cytotoxicity assay was used to detect the direct cytotoxicity of 139 malignant tumor patients and 355 healthy peripheral blood natural killer cells (NK cells) against K562 target cells labeled with 125IUdR. The results showed that the NK cell activity of tumor patients was significantly lower than that of healthy individuals. Among them, the decrease in NK activity in patients with hepatobiliary tumors and advanced tumors was particularly significant, and the NK activity of clinically cured patients returned to normal levels. The NK activity in patients with tumors at different clinical stages after surgical resection of tumors and improved with radiotherapy was significantly increased. The NK activity of healthy individuals declines with age, indicating that the weakening of non-specific immune surveillance is closely related to the occurrence and development of tumors.