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【目的】将抗虫基因转入栽培大豆中,提高大豆的抗食心虫能力。【方法】利用大豆未成熟子叶体细胞胚发生系统高效转化体系,将携带有人工合成的CryIA杀虫基因和CpTI基因的高效双价杀虫基因植物表达载体pGBI121S4ABC转化到大豆主栽品种吉林20与吉林27中。【结果】GUS活性分析、PCR、Southern blot检测证明,目的基因已整合到受体大豆基因组中。T1代转基因大豆抗虫测定表明,转基因材料虫食率比对照显著降低,抗虫能力显著提高。【结论】T3～T5连续3代接虫测定结果表明,转基因大豆株系0-195和0-150的虫食率均比对照低大约30%,其抗虫能力已基本稳定。
【Objective】 The insect-resistant genes were transferred into cultivated soybean to improve the resistance to soybean and beetle. 【Method】 The efficient transgenic system of soybean somatic embryogenesis system was used to transform pGBI121S4ABC, a plant expression vector with high efficiency and bivalent insecticidal gene encoding CryIA insecticidal gene and CpTI gene, into soybean cultivar Jilin 20 Jilin 27. 【Result】 The results of GUS activity assay, PCR and Southern blot showed that the target gene was integrated into the receptor soybean genome. The T1 generation of transgenic soybean pest resistance test showed that the insect-feeding rate of transgenic material was significantly lower than the control, pest resistance was significantly increased. 【Conclusion】 The results of T3 ~ T5 continuous generation 3 test showed that the insect-eating rates of transgenic lines 0-195 and 0-150 were both about 30% lower than that of the control, and their insect resistance was basically stable.