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8个花生品种SOD活性及热稳定性研究的结果表明:花生种子SOD活性高,每克鲜重含900~1600单位之间。在几个品种中,普通型(沭阳大站秧和沛县大拖秧)SOD活性最高,平均为1576.8U/gFW;珍珠豆型(赣榆小站秧和太仓拨藤)SOD活性较低,平均为954.5U/gFW。8个花生品种SOD同工酶谱相同,均为6条Cu·Zn-SOD带和1条Mn-SOD带。其中Mn-SOD以徐州68—4活性最高,为219.7U/gFW,其它几个品种Mn-SOD活性在56~100U/gFW。8个花生品种的SOD都具有较高的热稳定性,在90℃保温10min仍保留了22%~41%的活性,其中Mn-SOD热稳定性高于Cu·Zn-SOD。
The results of SOD activity and thermal stability of 8 peanut varieties showed that SOD activity of peanut seeds was high, with 900 ~ 1600 units per gram of fresh weight. In several cultivars, the SOD activity of the common type (Shuyang DaYanYang and Peixian DaYouJing) was the highest, with an average of 1576.8 U / gFW. The SOD activity of pearl bean (Ganyu XiaoYiJiao and Taicang DiaoTeng) was lower , An average of 954.5U / gFW. The SOD isozymes of the eight peanut cultivars were the same, with six Cu · Zn-SOD bands and one Mn-SOD band. The activity of Mn-SOD was the highest in Xuzhou 68-4, which was 219.7U / gFW. The activity of Mn-SOD in other varieties was 56-100U / gFW. SOD activity of 8 peanut varieties had higher thermal stability, and retained 22% -41% activity after 10 min incubation at 90 ℃. The thermal stability of Mn-SOD was higher than that of Cu · Zn-SOD.