Behet病患者结节红斑组织中CD40/CD40L共刺激分子的表达(英文)

• 作 者: 王红 杨培增 彭晓燕 赵萌 周红颜 黄祥坤
• 机 构: 中山大学中山眼科中心,中山大学中山眼科中心,首都医科大学附属北京同仁医院眼科中心,首都医科大学附属北京同仁医院眼科中心,中山大学中山眼科中心,中山大学中山眼科中心 1510060中国广东省广州市 10
• 出 处: 国际眼科杂志
• 发表日期: 2007年04期
• 关键词: Beh CD40/CD40L et Behet病 结节红斑 共刺激分子 单核巨噬细胞 抗原提呈细胞 erythema 双重染色

目的:探讨Behet病患者结节红斑中CD40/CD40L分子的表达及意义。方法:采用免疫组化方法对5例Behet病活动期患者腿或臂部结节红斑中CD4,CD8,CD19,CD68,HLA-DR,CD40和CD40L分子表达的情况进行了检测,并用双重染色方法探讨了细胞来源。结果:Behet病结节红斑中表皮约90%的细胞表达CD40,但无CD40L表达。真皮和皮下表达CD4,CD8,CD19,CD68,HLA-DR,CD1a,CD40L和CD40的细胞均显著增多。双染结果显示,45%的CD4+T细胞表达CD40L分子。100%CD68+细胞表达CD40分子。59.2%的HLA-DR+细胞表达CD40分子。结论:CD40L分子主要表达于CD4+T细胞,CD40分子主要表达于单核巨噬细胞、抗原提呈细胞和角朊细胞。CD40/CD40L通路可诱导巨噬细胞和T淋巴细胞的功能和活性,对Behet病患者结节红斑的发生、炎症的持续和扩大起重要作用。

Objective: To investigate the expression of CD40 / CD40L in erythema nodosum of Behet disease and its significance. Methods: The expressions of CD4, CD8, CD19, CD68, HLA-DR, CD40 and CD40L in 5 cases of active Behet disease were detected by immunohistochemistry. The staining method explored the source of the cells. RESULTS: About 90% of the cells in the epidermis of Behet’s disease nodules expressed CD40, but no CD40L expression. The number of cells expressing CD4, CD8, CD19, CD68, HLA-DR, CD1a, CD40L and CD40 subcutaneously increased significantly. Double staining results show that 45% of CD4 + T cells express CD40L molecules. 100% CD68 + cells express CD40 molecules. 59.2% of HLA-DR + cells expressed CD40 molecules. Conclusion: CD40L is mainly expressed on CD4 + T cells. CD40 molecules are mainly expressed on monocytes, antigen presenting cells and keratinocytes. The CD40 / CD40L pathway can induce the function and activity of macrophages and T lymphocytes and play an important role in the occurrence of erythema nodosum, persistence and expansion of inflammation in Behet disease.