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肌醇-3-磷酸合成酶(MIPS)基因是植酸合成代谢途径中重要的功能基因。本研究以两个大豆低植酸突变体Gm-lpa-TW-1、Gm-lpa-ZC-2以及其野生型亲本台湾75和浙春3号为材料,分析了MIPS1基因在其根、茎、叶、花和发育种子中转录水平上的表达特性。结果表明:在大豆发育的种子中MIPS1基因的表达表现为由弱到强再逐渐减弱的表达特性,在开花后22d的种子内,基因的表达达到高峰,此后逐渐减弱。突变体Gm-lpa-TW-1和野生型亲本台湾75的根、茎、叶和花中MIPS1基因的表达均较弱,但突变体Gm-lpa-TW-1的表达量略高于野生型亲本。与野生型亲本相比较,在开花后的22d,突变体Gm-lpa-TW-1发育种子中MIPS1基因的表达峰极显著的降低,仅为野生型的25%左右,而且在整个种子发育期间MIPS1基因的表达均较弱。突变体Gm-lpa-ZC-2和浙春3号的根、茎、叶和花中MIPS1基因的表达较弱,突变型和野生型之间没有显著的差异,但在种子发育的各个时期突变体Gm-lpa-ZC-2的表达量显著的高于野生型亲本浙春3号。在两个低植酸突变体发育的种子中MIPS1基因的表达与野生型亲本相比均发生了显著的变化,表明基因突变对MIPS1基因的表达均造成了显著的影响,在Gm-lpa-TW-1中表现为MIPS1基因表达的下调,而Gm-lpa-ZC-2中则表现为上调。
Inositol-3-phosphate synthase (MIPS) gene is an important functional gene in phytic acid metabolic pathway. In this study, two soybean low-phytic acid mutants Gm-lpa-TW-1, Gm-lpa-ZC-2 and their wild-type parents Taiwan 75 and Zhejiang Chun 3 were used to analyze the MIPS1 gene in its roots and stems , Leaf, flower and development of seed in the transcriptional level of expression. The results showed that MIPS1 gene expressed weakly to strongly and then gradually weakened in soybean seed. The expression of MIPS1 gene peaked at 22 days after anthesis, and then decreased gradually. The expression of MIPS1 gene in roots, stems, leaves and flowers of mutant Gm-lpa-TW-1 and wild-type parent Taiwan 75 was weak, but the expression level of Gm-lpa-TW-1 was slightly higher than that of wild type Parents. Compared with the wild-type parent, the expression of MIPS1 gene in the mutant Gm-lpa-TW-1 developing seed showed a very significant decrease at 22 days after flowering, which was only about 25% of the wild type. During the whole seed development MIPS1 gene expression are weak. The expression of MIPS1 gene in roots, stems, leaves and flowers of mutants Gm-lpa-ZC-2 and Zhechun 3 was weak, and there was no significant difference between mutant and wild type, but it was mutated in all stages of seed development The expression level of Gm-lpa-ZC-2 was significantly higher than that of the wild type Zhechun No.3. The expression of MIPS1 gene in two seedlings with low phytate mutation changed significantly compared with the wild-type parent, indicating that gene mutation had a significant effect on the expression of MIPS1 gene. In Gm-lpa-TW -1 showed down-regulation of MIPS1 gene expression, whereas Gm-lpa-ZC-2 up-regulated.