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AIM:To investigate the expression of cysteine-rich61(Cyr61),connective tissue growth factor(CTGF)and nephroblastomaoverexpressed gene(Nov)in hepatocellular carcinoma(HCC),and to evaluate the relationship between Cyr61,CTGF andNov genes expression with invasion and metastasis of HCC.METHODS:Thirty-one HCC specimens were divided intosmall hepatocellular carcinoma(SHCC),nodular hepatocellularcarcinoma(NHCC),solitary large hepatocellular carcinoma(SLHCC)according to their diameter and number ofnodes.Reverse transcription polymerse chain reaction(RT-PCR)was used to detect the mRNA expression levelsof Cyr61,CTGF and Nov genes in 31 resected specimensof hepatocellular carcinoma and para-cancerous normalliver tissues semi-quantitatively and the relation betweentheir expression levels and clinical pathological parameterswere compared.RESULTS:The expressions of Cyr61 and CTGF mRNA incarcinoma tissues were significantly higher than those inpara-cancerous normal liver tissues(P<0.01).Theexpressions of Cyr61 and CTGF mRNA in HCC with venousinvasion were higher than those in HCC without venousinvasion.CTGF expression in HCC Edmondson’s grade Ⅲ-Ⅳ was significantly higher than that in HCC Edmondson’sgradeⅠ-Ⅱ(P=0.022).There was no obvious correlationbetween Nov mRNA and clinical-pathological features.Compared to NHCC,SLHCC had better cell differentiation,easier capsule formation,less microscopic venous invasion,milder liver cirrhosis.The expressions of Cyr61 and CTGFmRNA in NHCC were significantly higher than those inSLHCC and SHCC.CONCLUSION:Cyr61 and CTGF genes may play an importantrole in hepatocellutar carcinogenesis and correlate withrecurrence and metastasis of hepatocellular carcinoma.SLHCC has better biological behaviors than NHCC.
To investigate the expression of cysteine-rich61 (Cyr61), connective tissue growth factor (CTGF) and nephroblastomaoverexpressed gene (Nov) in hepatocellular carcinoma (HCC), and to evaluate the relationship between Cyr61, CTGF andNov genes expression with invasion and metastasis of HCC.METHODS: Thirty-one HCC specimens were divided intosmall hepatocellular carcinoma (SHCC), nodular hepatocellular carcinoma (NHCC), solitary large hepatocellular carcinoma (SLHCC) according to their diameter and number of nodes. was used to detect the mRNA expression levels of Cyr61, CTGF and Nov genes in 31 resected specimens of hepatocellular carcinoma and para-cancerous normalliver tissues semi-quantitatively and the relation betweentheir expression levels and clinical pathological parameterswere compared .RESULTS: The expressions of Cyr61 and CTGF mRNA The incarcinoma tissues were significantly higher than those in para-cancerous normal liver tissues (P <0.01). Theexpression s of Cyr61 and CTGF mRNA in HCC with venous invasion were higher than those in HCC without venous invasion. CTGF expression in HCC Edmondson’s grade III-IV was significantly higher than that in HCC Edmondson’s grade I-II (P = 0.022). There was no obvious correlation between Nov mRNA and clinical-pathological features. Compared to NHCC, SLHCC had better cell differentiation, easier capsule formation, less microscopic venous invasion, milder liver cirrhosis. The expressions of Cyr61 and CTGF mRNA in NHCC were significantly higher than those in SLHCC and SHCC.CONCLUSION : Cyr61 and CTGF genes may play an importantrole in hepatocellutar carcinogenesis and correlate with recur and metastasis of hepatocellular carcinoma. LHHCC has better biological behaviors than NHCC.