C-erbB-2在乳腺良恶性病变表达及与DNA含量和细胞形态的相关性研究

• 作 者: 师永红 孙慧宽 宫丽平 李增山
• 机 构: 内蒙古医学院病理教研室
• 出 处: 中国体视学与图像分析
• 发表日期: 1998年02期
• 关键词: 乳腺良恶性病变 C-erbB-2 细胞生长 乳腺肿瘤 DNA 免疫组化染色 细胞形态参数 癌基因激活 乳腺良性病变 图像分析技术

本文利用免疫组化（ＬＳＡＢ）法，Ｆｅｕｌｇｅｎ染色及图像分析技术对乳腺癌８４例和乳腺良性病变２２例进行检测。结果：（１）ＣｅｒｂＢ２蛋白免疫组化染色的着色面积与着色深度高度相关（Ｐ＜００１）。（２）ＣｅｒｂＢ２阳性表达全部为恶性肿瘤，总表达率为３３％。在不同年龄、肿瘤分级以及淋巴转移情况之间ＣｅｒｂＢ２阳性表达率差别无统计学意义。（３）ＣｅｒｂＢ２免疫组化染色的平均光密度与ＤＮＡ倍体水平高度正相关（Ｐ＜００１）。（４）ＣｅｒｂＢ２阳性肿瘤的细胞形态参数均显著大于阴性肿瘤。结论∶乳腺良恶性病变中，ＣｅｒｂＢ２癌基因激活仅见于乳腺癌，对乳腺癌的诊断有意义，对预后判断只起辅助作用

In this paper, 84 cases of breast cancer and 22 cases of benign breast lesions were detected by immunohistochemistry (LSAB), Feulgen staining and image analysis techniques. Results: (1) The staining area of ​​CerbB2 protein immunohistochemical staining was highly correlated with the depth of staining (P<001). (2) The positive expression of CerbB2 was all malignant and the total expression rate was 33%. There was no significant difference in the positive expression rate of C-erbB-2 between different ages, tumor grade, and lymphatic metastasis. (3) The average optical density of CerbB2 immunohistochemical staining was positively correlated with DNA ploidy level (P<001). (4) The cell morphological parameters of CerbB2 positive tumors were significantly greater than those of negative tumors. Conclusion: In breast benign and malignant lesions, CerbB2 oncogene activation is only found in breast cancer. It is meaningful for the diagnosis of breast cancer and only plays a role in determining prognosis.